Selection and Evaluation of Reference Genes for Quantitative Real-Time PCR in Tomato (Solanum lycopersicum L.) Inoculated with Oidium neolycopersici

In order to screen out the most stable reference genes in tomatoes under powdery mildew (PM) stress and study expression of related interaction between tomato PM more accurately, this will provide a calibration basis for functional genes. study, stabilities eight candidate EF1α, L33, Act, Ubi, GAPDH, UK, CAC TIP41 susceptible resistant were ranked using four different computation programs, including geNorm, Normfinder, BestKeeper comparative ∆CT method. Then RefFinder was used analyze ranking results kinds software comprehensively. Finally, selected validated by target gene SlMLO1. The geNorm showed that normalization qRT-PCR two could meet requirements. comprehensive analysis Act EF1α both varieties. combination GAPDH ‘MM’. ‘62579′. Generally, varieties stress. This lay foundation research